Tuesday, December 31, 2019
How Fiber Optics Was Invented
Fiber optics is the contained transmission of light through long fiber rods of either glass or plastics. The light travels by process of internal reflection. The core medium of the rod or cable is more reflective than the material surrounding the core. That causes the light to keep being reflected back into the core where it can continue to travel down the fiber. Fiber optic cables are used for transmitting voice, images, and other data at close to the speed of light. Who Invented Fiber Optics? Corning Glass researchersà Robert Maurer, Donald Keck, and Peter Schultzà invented fiber optic wire or Optical Waveguide Fibers (patent #3,711,262) capable of carrying 65,000 times more information than copper wire, through which information carried by a pattern of light waves could be decoded at a destination even a thousand miles away.à Fiber optic communication methods and materials invented byà them opened the door to the commercialization of fiber optics. From long-distance telephone service to the internet and medical devices such as the endoscope, fiber optics are now a major part of modern life.à Timeline 1854: John Tyndall demonstrated to the Royal Society that light could be conducted through a curved stream of water, proving that a light signal could be bent.1880:à Alexander Graham Bell invented his Photophone, which transmitted a voice signal on a beam of light. Bell focused sunlight with a mirror and then talked into a mechanism that vibrated the mirror. At the receiving end, a detector picked up the vibrating beam and decoded it back into a voice the same way a phone did with electrical signals. However, many things ââ¬â a cloudy day, for instance ââ¬â could interfere with the Photophone, causing Bell to stop any further research with this invention.1880: William Wheeler invented a system of light pipes lined with a highly reflective coating that illuminated homes by using light from an electric arc lamp placed in the basement and directing the light around the home with the pipes.1888: The medical team of Roth and Reuss of Vienna used bent glass rods to illuminate bod y cavities.1895: French engineer Henry Saint-Rene designed a system of bent glass rods for guiding light images in an attempt at early television.1898: American David Smith applied for a patent on a bent glass rod device to be used as a surgical lamp.1920s: Englishman John Logie Baird and American Clarence W. Hansell patented the idea of using arrays of transparent rods to transmit images for television and facsimiles respectively.1930: German medical student Heinrich Lamm was the first person to assemble a bundle of optical fibers to carry an image. Lamms goal was to look inside inaccessible parts of the body. During his experiments, he reported transmitting the image of a light bulb. The image was of poor quality, however. His effort to file a patent was denied because of Hansells British patent.1954: Dutch scientist Abraham Van Heel and British scientist Harold H. Hopkins separately wrote papers on imaging bundles. Hopkins reported on imaging bundles of unclad fibers while Van He el reported on simple bundles of clad fibers. He covered a bare fiber with a transparent cladding of a lower refractive index. This protected the fiber reflection surface from outside distortion and greatly reduced interference between fibers. At the time, the greatest obstacle to a viable use of fiber optics was in achieving the lowest signal (light) loss.1961: Elias Snitzer of American Optical published a theoretical description of single-mode fibers, a fiber with a core so small it could carry light with only one waveguide mode. Snitzers idea was okay for a medical instrument looking inside the human, but the fiber had a light loss of one decibel per meter. Communications devices needed to operate over much longer distances and required a light loss of no more than ten or 20 decibels (a measurement of light) per kilometer.1964: A critical (and theoretical) specification was identified by Dr. C.K. Kao for long-range communication devices. The specification was ten or 20 decibels o f light loss per kilometer, which established the standard. Kao also illustrated the need for a purer form of glass to help reduce light loss.1970: One team of researchers began experimenting with fused silica, a material capable of extreme purity with a high melting point and a low refractive index. Corning Glass researchers Robert Maurer, Donald Keck, and Peter Schultz invented fiber optic wire or Optical Waveguide Fibers (patent #3,711,262) capable of carrying 65,000 times more information than copper wire. This wire allowed for information carried by a pattern of light waves to be decoded at a destination even a thousand miles away. The team had solved the problems presented by Dr. Kao.1975: The United States government decided to link the computers at the NORAD headquarters at Cheyenne Mountain using fiber optics to reduce interference.1977: The first optical telephone communication system was installed about 1.5 miles under downtown Chicago.à Each optical fiber carried the e quivalent of 672 voice channels.By the end of the century, more than 80 percent of the worlds long-distance traffic was carried over optical fiber cables and 25 million kilometers of the cable. Maurer, Keck, and Schultz-designed cables have been installed worldwide. U.S. Army Signal Corp The following information was submitted by Richard Sturzebecher. It was originally published in the Army Corp publication Monmouth Message. In 1958, at the U.S. Army Signal Corps Labs in Fort Monmouth New Jersey, the manager of Copper Cable and Wire hated the signal transmission problems caused by lightning and water. He encouraged Manager of Materials Research Sam DiVita to find a replacement for copper wire. Sam thought glass, fiber, and light signals might work, but the engineers who worked for Sam told him a glass fiber would break. In September 1959, Sam DiVita asked 2nd Lt. Richard Sturzebecher if he knew how to write the formula for a glass fiber capable of transmitting light signals. DiVita had learned that Sturzebecher, who was attending the Signal School, had melted three triaxial glass systems using SiO2 for his 1958 senior thesis at Alfred University. Sturzebecher knew the answer. While using a microscope to measure the index-of-refraction on SiO2 glasses, Richard developed a severe headache. The 60 percent and 70 percent SiO2 glass powders under the microscope allowed higher and higher amounts of brilliant white light to pass through the microscope slide and into his eyes. Remembering the headache and the brilliant white light from high SiO2 glass, Sturzebecher knew that the formula would be ultra pure SiO2. Sturzebecher also knew that Corning made high purity SiO2 powder by oxidizing pure SiCl4 into SiO2. He suggested that DiVita use his power to award a federal contract to Corning to develop the fiber. DiVita had already worked with Corning research people. But he had to make the idea public because all research laboratories had a right to bid on a federal contract. So in 1961 and 1962, the idea of using high purity SiO2 for a glass fiber to transmit light was made public information in a bid solicitation to all research laboratories. As expected, DiVita awarded the contract to Corning Glass Works in Corning, New York in 1962. Federal funding for glass fiber optics at Corning was about $1,000,000 between 1963 and 1970. Signal Corps Federal funding of many research programs on fiber optics continued until 1985, thereby seeding this industry and making todays multibillion-dollar industry that eliminates copper wire in communications a reality. DiVita continued to come to work daily at the U.S. Army Signal Corps in his late 80s and volunteered as a consultant on nanoscience until his death at age 97 in 2010.
Monday, December 23, 2019
A Medical Revolution Essay - 1547 Words
In the spring of 1918, the first wave of one of the deadliest influenza pandemics began plaguing its victims (Peters, ix). Over the span of three lethal waves, the pandemic claimed approximately forty million victims, eradicating nearly twenty percent of the entire worldââ¬â¢s population, or about one out of five individuals (Peters, ix). To make matters more dire, the ill-suited medical community was exceptionally unprepared for such a wide-scale pandemic: Doctors had very basic tools, knew little about diseases, and had no experience with vaccinations or prevention (Peters, 1-5; ââ¬Å"The 1920s: Medicine and Health: Overviewâ⬠, n.p.). People blindly faced the epidemic, relying on folk remedies such as consuming wine, drinking antiseptic, andâ⬠¦show more contentâ⬠¦Although doctors were supposed to be considered medical experts, they were not taken seriously due to the fact that a patient had less than a fifty percent chance of benefiting from a doctorââ¬â¢s vi sit (ââ¬Å"The 1920s: Medicine and Health: Overviewâ⬠, n.p.). Doctors struggled to diagnose and fix medical problems due to inexperience and lack of tools (ââ¬Å"The 1920s: Medicine and Health: Overviewâ⬠, n.p.). As a result of the substandard medical community, maternal deaths and child deaths were commonplace, while simple sicknesses often turned into fatal infections (ââ¬Å"The 1920s: Medicine and Health: Overviewâ⬠, n.p.). When the first wave of the influenza pandemic struck in the spring of 1918, the medical community was taken aback by the pandemicââ¬â¢s unpredicted wrath (Peters, 13). The typically mellow influenza virus, characterized by familiar symptoms of fever, headache, fatigue, cough, sore throat, congestion, and body aches, claimed victims within hours (Peters, ix, 1-5). This was a sickness like no other, and it left the medical community baffled. Doctors nor medical experts knew what the sickness was, why it was spreading, what was causing it, or how to fix it (Peters, 1-5). All branches of the medical field practically shut down: researchers found no potentialShow MoreRelatedIndustrial Revolution Essay799 Words à |à 4 PagesWas the Industrial Revolution a blessing or a curse? à à à Starting in 18th century Great Britain, the Industrial Revolution, sparked a change in industry that is still present today. The Industrial Revolution was a great blessing to society, with innovations in consumer goods, medicine, housing and sanitation, the revolution changed the course of history for the good. 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Add a mustache and a beard to the Mona Lisa--it becomes L.H.O.O.Q., a Dada piece of art by Marcel Duchamp. Assemble a bicycle seat and handlebars--it becomes Bull s Head, a found object artwork by Pablo Picasso. The creation of such works, which directly borrow ideas or actual parts of another work, can be considered plagiarism, but that assumptionRead MoreThe Effects And Effects Of The Industrial Revolution During The Victorian Era1465 Words à |à 6 Pagesthe Victorian years, some of the main characteristics we think of the industrial revolution and the effects during the Victorian period. In this assignment Iââ¬â¢m going to explain the impact that the industrial revolution had on people in There are three main characteristics of the industrial revolution which most people would think of first which are urbanization, suburb slums and agricultural revolution. 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Saturday, December 14, 2019
Strategic Alliance Between Nokia and Microsoft Free Essays
Business Administration knowledge assists the implementation of the strategic alliance between Nokia and Microsoft from several aspects. To begin with, decisions about leadership are one of the most disturbing problems in the strategic alliance; interim leaders are appropriate solutions to the issue (Werther, 1998). Interim leaders are those haired from a third party, not belonging to the alliance partners. We will write a custom essay sample on Strategic Alliance Between Nokia and Microsoft or any similar topic only for you Order Now Compared to selecting a leader from one of the partners, interim leaders have their advantages. It eliminates the assumption from both the public and the employees that one party has the domination, which assists to build a neutral image of the alliance (Werther, 1998). Therefore, Nokia and Microsoft are in a fairly equivalent position in the alliance. Moreover, their focus is the success of the alliance rather than the benefits of one of the alliance parties (either Nokia or Microsoft). Compared to outside consultants, interim leaders also have more merits. Werther (1998) suggests that interim leaders have operational or practical authority instead of advising authority owned by consultants, and they are suitable in the start-up stage of the alliance. As the alliance between Nokia and Microsoft has just begun, interim leaders can be helpful. Interim leaders contribute to distinguishing between ââ¬Å"actual values that are ââ¬Ëin useââ¬â¢ from stated or ââ¬Ëespousedââ¬â¢ values that are merely touted for internal or external consumptionâ⬠(Argyriset al, 1985; cited in Werther, 1998, p. 342). Thus, Nokia and Microsoft should select an interim leader from the third party rather from themselves. In addition, information flow management including intellectual property protection is crucial to the success of the alliance. Microsoft is the leading software company in the world, and Nokia has been the leading phone manufacturer all over the world. Thus, the value of intangible information can be even greater than tangible assets companies own. However, partners are often in a dilemmatic situation where they want to balance the outflow of information to successfully achieve the task and the protection of intellectual asset (Osborn et al, 2001). The careful management of information is of great importance. Firstly, alliance managers need to have a clear understanding of partnerââ¬â¢s intention of learning and the extent to which partners are willing to learn from cooperators (Ireland et al, 2002). Then, suitable organizational control like ââ¬Å"integrating mechanisms and use of interest-aligning incentive plansâ⬠(Geringer Herbert, 1989; Kumar Seth, 1998; cited in Ireland et al, 2002, p. 437) can be used to manage information flow. When it comes to intellectual property protection, both Microsoft and Nokia should think about the value of the companyââ¬â¢s intellectual asset and decide the key components that belong to inadvertent transfer. In this case, Microsoft may need to have a consideration of the safety of core techniques involved in providing the operating system. Moreover, Gadieshet al (2002) emphasize the necessity to have an intellectual property audit internally before alliance, with companies whose central capital consist of intellectual property. Lastly, building trust between partners also contributes to the success of strategic alliance. Trust means that the partnerââ¬â¢s behaviours will meet expectations, and it leads partners to bear risks and produces a positive effect on the alliance (Ireland et al, 2002). Continuing to show the companyââ¬â¢s goals for strategic alliance while partners do the same and demonstrate patience are important actions in building trust (Cullen et al, 2000; cited in Ireland et al, 2002, p. 38). As the problems are usually clearer to middle managers and engineers who experience every day alliance activities (Osborn et al, 2001), thus, the trust and communication building between these people from Microsoft and Nokia cannot be ignored. All in all, experts and managers with sophisticated business administration knowledge are required to implement these tasks. The successes of these aspects contribute to the success of the strategic alliance. How to cite Strategic Alliance Between Nokia and Microsoft, Papers
Friday, December 6, 2019
A Balanced Budget Essay Example For Students
A Balanced Budget? Essay A Balanced Budget?This year President Clinton will submit his proposed legislation for theFederal Budget to Congress. The fact that we have divided government (ex.,Democratic President, Republican majority in Congress) means the majority ofthat legislation wont make it through the first ten minutes of a Congressionalsession. The President in turn will veto legislation presented to him byCongress. The whole situation is a vicious, never ending circle. Each side islooking out for their own best interests, and after years, even decades of thisthe United States has a huge budget deficit. Is there a solution to all thismadness? Is it feasible to balance the Federal Budget? Every politician onCapitol Hill claims to have the answer. The Federal Government goes as far toemploy some of the most renowned economists in the world to try to solve thedeficit mess, and they still havent figured it out. The budget simulation exercise by The Committee for a ResponsibleFederal Budget provided choices Congress has to use as its guide for theupcoming year. How hard can it be to balance the budget I thought? After doingthe exercised I realized the title of the simulation exercise, The FY 1997Budget: An Exercise in Hard Choices, could not have been more appropriate. Itis possible though to balance the Federal Budget, provided you follow 3 simplerules. First you must decide what you feel is important, then cut withoutconsciousness, and if that doesnt work, alter your baseline. Important ChoicesWhen deciding on what I thought was important to protect in the budget,I felt like a politician myself. I protected my own self interests. First up wasDefense. The fact that I am in the Marine Officer Program weighed heavily on mydecision to increase Defense spending by 17.6 billion (all amounts in billions,unless noted), following the Congressional Budget Resolution. The Presidentsplan just didnt provide enough capital, the Congressional BlackCaucus/Progressive Caucus would basically wipe out my career before it evenstarts, and I just can not have that. Next up is Education. Without the directstudent loan program, I would have no way to fund my education other than goingto some bank and going through the demeaning process of begging a loan officerto lend me money for school. I feel a sense of loyalty to the President on thisissue, because without this program I would still be doing concrete constructionback home in Indiana. As a result, I voted to increase Education al spending33.4.My other concern or problem area is Administration of Justice. Crime is wayout of control in this country. There has to be an end. I voted to increasespending 23.6, opting for the Presidents budget because it provides enoughcapital to temporarily combat the problem. You might be thinking, wow this fooljust increased the deficit by 74.6, but unless are borders are protected, arechildren educated, and until we can feel safe in our homes at night, thiscountry wont realize its potential. International ; Domestic SpendingAt this point, the deficit stands at 883.6 (809 billion beginning +current spending). It is time to cut the waste. First, International Affairsrequires attention. Does the United States really need to fund every countrysstruggle? I dont think so, so I agree with the Budget Resolution ConferenceAgreement (BRCA), and I vote to cut 12.4. Next on the list, General Science, Space and Technology. I vote todiscontinue the Space Station Program. As the report says, scientists have lostinterest so why keep funding it. Another 11.2 by the wayside. The Energy issue is a complex one, but nevertheless the DOE has to go. Jackie Robinson EssayUnfortunately there happens to be some programs that need money. First,I chose to spend 117 billion on giving tax credits for Families/Children, optingfor the Presidents budget. I voted to repeal the 4.3 cent motor fuels tax,spending 2.9. Finally, I spent 15.6 to Provide Tax Incentives for Long TermCare Insurance. All this spending on revenues cost me 187.2 billion. My rationalbehind my spending, is once again in my own self interest, for if I was a Memberof Congress, with all the cuts I have made, I have to give back a little. Balanced?Will the budget balance? Before the exercise began there was an 809billion deficit. I spent 209.3 billion, on a total of 6 different programs. Icut 1048.7 billion from 18 different recipients of federal dollars. This amountsto a balanced budget. Total Deficit Reduction from Policy changes equaled838.7(all amounts in billions). Interest savings amounted to 83.87. Totaldeficit reduction, 992.57. Add in the baseline budget deficit of 809 billionfrom the previous year. 113.57 remains. Policy changes totaled at 718.7. Spending changes as a percentage ended up 85.7%. Finally revenue changesfinished at 14.31%. Is it feasible to balance the Federal Budget? It is if it is a game orassignment. Dealing with issues that effect individuals from all walks of lifeis almost impossible. I cut all but a few categories under International Domestic Spending. It is not realistic in the real world. Cutting funding forone program not only effects those involved, but inadvertently effects others. An example would be cutting welfare benefits. With no money, no job, and nofuture prospects, an individual might result to crime, whether selling narcoticsor robbery, in order to support their family. The decisions that the Presidentmakes in preparing a budget have to be overwhelming. In Congress, individualdecisions are more anonymous. The records are accessible, but who reallyremembers how an individual Member of Congress voted. How many taxpayers knowwhat a baseline is. The President and Congress each uses their own baseline, ithelps them justify spending or cuts. Until the President and Congress can agreeto bal ance the budget, cut waste, and quit talking about it, there will be adeficit. Works CitedExercise In Hard Choices. Committee for a Responsible Federal Budget. July 1996. History
Friday, November 29, 2019
James Hetfield Essays - Metallica, Ron McGovney, Leather Charm
James Hetfield James Hetfield the American Dream James is probably the driving creative force. - Kirk Hammett Hetfield brings the pride. He stands a very tall and strong guy over all of, not just the music, but all of what is Metallica, he's been through a lot of shi* but still pushed to get where he is today.? - Jason Newstead Although the Americans dream, weather new or old to this country is to succeed financially, to some it is to become well known to your fellow man. The idea of crawling out of the gutter with nothing but your pride and earning respect from everyone who had doubted you before. Since the birth of this equal opportunity nation, people from all aspects of cultures and countries have strived through thick and thin to surpass their fellow man and better themselves in the eyes of society. The American dream does not just occur with citizens new to our country but to generations upon generations of people whose family tree had begun anew when their ancestors first arrived to this land. This theory proves itself valid with a number of successful people who have never had the opportunity of experiencing ?old wealth?. They are ridiculed for their dreams and made fun of for their ideas, but in the end these prospects of society made it to the top with the occasional supporter and Hetfield was born to a truck driver and light opera singer on August 3rd, 1963, in Los Angeles. His family's Christian Science religious beliefs are often mentioned as the root of James' tortured soul lyrics. Musically, he began at age 9 with piano lessons, then banging away on his brother David's drums and finally to guitar. With his guitar in hand, James aspired to become a rock star in his first band, Obsession. After seeing AC/DC perform in Los Angeles on august 14th 1977The band was made up of the Veloz brothers on bass and drums and Jim Arnold on guitar. A pair of friends, Ron McGovney and Dave Marrs, acted as the band's roadies. This meant sitting in the loft of the Veloz garage running a control panel for makeshift lighting effects. After Obsession broke up, the Marrs-Hetfield-McGovney trio continued jamming together. Phantom Lord was James Hetfield's second band. After moving to La Brea, James attended Brea Olinda High School and met up with drummer Jim Mulligan. The two would jam at lunchtime with another guitar player, but eventually scared him away with their loud and heavy sounds. Hugh Tanner was discovered carrying around part of a flying V guitar at school. And shortly thereafter, Phantom Lord was born. With Hugh on guitar and Mulligan on drums, James sang and played guitar. The group went through a few bass players until graduation when James moved back to Downey. In Downey, James moved into a house owned by Ron McGovney's parents that was slated for demolition due to an expressway expansion. This house was the perfect place for James and Ron to crash and hold rehearsal and practice jams. James talked Ron into taking up bass, and promised to teach him... And as Ron joined the group, Phantom Lord was no more. They were now Leather Charm. James Hetfield's third band overall, Leather Charm was formed from the members of Phantom Lord, except that James performed sole singing duties (no guitar) and Ron was added to play bass guitar. Completing the line-up were Phantom Lord carry-overs Hugh Tanner and Jim Mulligan. Leather Charm was more of a glammy attempt at music. The band played originals and covers like Quiet Riot's Slick Black Cadillac, Iron Maiden's Remember Tommorrow and a few others. The band managed to perform at a few parties and recorded a demo, but then began to fall apart. *Picture*First Tanner left the band and was replaced by Troy James. Then Mulligan left for a more progressive, Rush-like band. With no drummer, the band was forced to call it quits. Although it was Mulligan's departure that led to James searching for a new drummer, it was Hugh Tanner that introduced James to Lars Ulrich. Ultimately it was Leather Charm's break up, through Jim Mulligan's quitting, that led James and Lars to meet... and to form Metallica. From the
Monday, November 25, 2019
Cause and Effect Outline Practice Exercise
Cause and Effect Outline Practice Exercise Here well practice making a simple outline: a list of the key points in a paragraph or essay. This basic outline can help us revise a composition by showing at a glance if we need to add, remove, change, or rearrange any supporting details. Why Outlines are Useful Some writers use outlines to develop a first draft, but this approach can be tricky: how can we organize our information before weve figured out what we want to say? Most writers need to start writing (or at least freewriting) in order to discover a plan. Whether you use an outline for drafting or revising (or both), you should find it a useful way to develop and organize your ideas in paragraphs and essays. Cause and Effect Paragraph Lets begin by reading a students cause-and-effect paragraph, Why Do We Exercise?, and then well arrange the students key points in a simple outline. Why Do We Exercise? These days, just about everyone, from toddler to retiree, seems to be running, pedaling, lifting weights, or performing aerobics. Why are so many people exercising? There are several reasons. Some people, the ones in designer jump suits, exercise simply because keeping in shape is trendy. The same people who a few years ago thought doing drugs was cool are now just as seriously involved in self-conditioning. Other people exercise to lose weight and appear more attractive. The paunchy crowd is willing to undergo extreme self-torture in the name of beauty: thin is in. Finally, there are those who exercise for their health. Regular, intensive exercise can strengthen the heart and lungs, build endurance, and improve the bodys immunity system. In fact, judging from my observations, most people who exercise probably do so for a combination of these reasons. Cause and Effect Paragraph Outline Now heres a simple outline of the paragraph: Opening: Everyone is exercising.Question: Why are so many people exercising?Reason 1: Be trendy (exercise is cool)Reason 2: Lose weight (thin is in)Reason 3: Stay healthy (heart, endurance, immunity)Conclusion: People exercise for a combination of reasons. As you can see, the outline is just another form of listing. The opening and question are followed by three reasons, each expressed in a brief phrase and followed in parenthesis by an equally brief explanation. By arranging the main points in a list and using key phrases rather than complete sentences, we have reduced the paragraph to its basic structure. Cause and Effect Outline Exercise Now try it yourself. The following cause-and-effect paragraph, Why Do We Stop at Red Lights?, is followed by the plan for a simple outline. Complete the outline by filling in the main points given in the paragraph. Why Do We Stop at Red Lights? Say its two in the morning with not a policeman in sight, and you approach an empty intersection marked by a red light. If youre like most of us, you stop and wait for the light to turn green. But why do we stop? Safety, you might say, though you can see perfectly well that its quite safe to cross. Fear of being nabbed by a sneaky police officer is a better reason, but still not very convincing. After all, the police dont generally make a habit of setting up road traps in the dead of night. Perhaps we are just good, law-abiding citizens who wouldnt dream of committing a crime, even though obeying the law in this case does seem faintly ridiculous. Well, we may claim to be following the dictates of our social conscience, but another, less high-minded reason probably underlies it all. We stop at that red light out of dumb habit. We probably dont consider whether its safe or unsafe to cross, right or wrong; we stop because we always stop at red lights. And, of course, even if we were to think about it as we idled there at the intersection, the light would probably turn green before we could come up with a good reason for why we do what we do. Fill out the simple outline for Why Do We Stop at Red Lights?: Opening: __________Question: __________?Reason 1: __________Reason 2: __________Reason 3: __________Reason 4: __________Conclusion: __________ Completed Cause and Effect Outline Now compare your outline with the completed version of the simple outline for Why Do We Stop at Red Lights? Opening:à Red light at two a.m.Question:à Why do we stop?ââ¬â¹Reason 1:à Safety (though we know its safe)Reason 2:à Fear (though police arent around)Reason 3:à Social conscience (maybe)Reason 4:à Dumb habit (most likely)Conclusion:à We have no good reason. Once you have practiced creating a few simple outlines, youre ready to move on to the next step: evaluating the strengths and weaknesses of the paragraph you have outlined.
Thursday, November 21, 2019
Peroxisomes research Article Example | Topics and Well Written Essays - 1250 words
Peroxisomes research - Article Example Luciferase measurement The genomic DNA was amplified using PCR 500 bp upstream region of Act1 Cta1, iPex3A and iPex3B open reading frames. The PCR fragments were inserted upstream of Luciferase reporter gene in pLUC plasmid. The promoter-pLUC plasmids were transformed into wild-type yeast via the PEG - LiAC transformation method. The Transformants were plated on YPD plates with kanamycin. Each colony of the transformant was grown in 5ml YPD for 18 hours at 30C. The cells were spin down in a centrifuge. The cells were resuspended in 1mL low fluorescence media (YNB). This sample was divided into two parts. To one sample 100uls of 0.003% hydrogen peroxide was added. The tubes were incubated at 28C for 30 minutes. The cells were spin down. The cells were washed 3 times with 1ml 1XPBS. The final pellet was resuspended in 500 ls of 1X PBS. The cells were separated into 10,000 cell aliquots. One ml lysis reagent to the cells and incubated for 5 mins. The luciferase assay reagent was added to lysed cells. The int ensity of light emitted from sample was measured with a luminometer. Statistical analysis: Each sample were measured six times and standard deviation was determined. Further, t-test were carried out to signify the difference in the values. Results: To determine the role of iPEX3A and iPAX3B in peroxisomal function set of experiments were carried out. In first sets of experiments accumulation of H2O2 were determined in different mutant having single mutation in cat1, ipex3A and ipex3B. Table 1 displays the results of H2O2 treatment on these three mutants along with wild type (positive control) cells. There was significant increase (p
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